Creating hybridomas - A Guide to Generating Antibody-Producing Cell Lines摘要：Creating hybridomas - A Guide to Generating Antibody-Producing Cell LinesIntroduction
Creating hybridoma cells is an indispensable technique widely used in the
Introduction
Creating hybridoma cells is an indispensable technique widely used in the field of immunology to produce monoclonal antibodies with high specificity and affinity. This technique is based on the fusion of myeloma cells and antibody-secreting plasma cells, resulting in the formation of hybridomas. In this article, we will explore the steps involved in generating hybridomas through the fusion of immune cells with myeloma cells.Generation of Immune Cells and Myeloma Cells
The very first step in generating hybridomas is the preparation of immune cells and myeloma cells. Immune cells are obtained from animals by triggering an immune response against the antigen of interest. After obtaining the splenocytes or lymphocytes, the immune cells are typically purified by density gradient centrifugation. In contrast, myeloma cells, which are taken from immortalized myeloma cell lines, are cultured separately.Hybridoma Formation
Screening of Hybridomas
After the generation of hybridoma cells, the next step is to screen the cells to identify clones that produce antibodies targeting the antigen of interest. ELISA or flow cytometry can be used to screen hybridoma cells. Once identified, the antibodies produced by the hybridomas can be purified from the cell culture supernatant using various chromatographic techniques.Conclusion
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